{"title":"Microbiology Stain Kits","description":"","products":[{"product_id":"chlorazole-black-e-ci-30235-certified","title":"Chlorazole black E, C.I. 30235, certified","description":"\u003cp\u003eA valuable stain in general histology and cytology. Gives sharp, clear-cut pictures of both nuclei and cytoplasmic structures. Also useful for differentiation of fungi.\u003c\/p\u003e","brand":"Polysciences","offers":[{"title":"02730-25 (25 g)","offer_id":46425675301078,"sku":"02730-25","price":147.45,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0713\/5258\/2358\/files\/logo_only_d9409bb0-c6d4-4f03-a9c6-0ddb4c53990c-325648.jpg?v=1737983001"},{"product_id":"szechrome-nas","title":"Szechrome NAS","description":"\u003cp\u003eSuitable for the determination of the nitrate content of natural waters, industrial waste effluents, soil, plant and meat extracts, tinned goods, biological fluids (sputum, urine), chemicals, fertilizers and drugs. NAS has been used in place of the phenoldisulfonic acid, brucine and chromotropic acid methods used previously.\n\u003cbr\u003eThis sensitive reagent reacts rapidly to produce stable colors suitable for precise quantitative determinations or rapid estimations in routine field work. Szechrome NAS produces color reactions which are specific and proportional to the nitrate content of the sample tested.\n\u003cbr\u003eAdvantages\u003c\/p\u003e\n\n\u003cp\u003e  Stable\n\u003cbr\u003e  Non-toxic\n\u003cbr\u003e  Stable color develops rapidly and is not temperature sensitive\u003c\/p\u003e\n\n\u003cp\u003eOptical density (OD) at 570nm after 5 min. development is proportional to nitrate in the range of 2-20ppm.\u003c\/p\u003e","brand":"Polysciences","offers":[{"title":"08762-5 (5 g)","offer_id":46425697812694,"sku":"08762-5","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0713\/5258\/2358\/files\/08762_8e466637-a7a9-471a-acdf-82ca46bfffc0-612846.jpg?v=1737984161"},{"product_id":"cyanoditolyl-tetrazolium-chloride-ctc","title":"Cyanoditolyl Tetrazolium Chloride (CTC)","description":"\u003cdiv class=\"pdp-content-block\"\u003e\n\u003cdiv class=\"pdp-eyebrow\"\u003e⟡ Microbiology \u0026amp; Cell Analysis \/ Redox \u0026amp; Viability Assays\u003c\/div\u003e\n\u003ch2\u003eCyanoditolyl Tetrazolium Chloride (CTC)\u003c\/h2\u003e\n\u003cp\u003eCyanoditolyl tetrazolium chloride (CTC) is a redox-sensitive tetrazolium dye widely used to assess cellular metabolic activity and respiration. It is commonly applied in microbiology and cell biology workflows to identify actively respiring cells through the formation of a fluorescent formazan product.\u003c\/p\u003e\n\u003cp\u003eCTC is frequently used for the direct epifluorescent enumeration of respiring bacteria in environmental, food, and marine samples. Upon reduction by components of the electron transport chain, CTC forms an insoluble, fluorescent formazan that accumulates within cells, allowing researchers to distinguish metabolically active populations using fluorescence microscopy.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3\u003eKey Features \u0026amp; Benefits\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRedox indicator dye:\u003c\/strong\u003e measures cellular respiration and metabolic activity\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDirect detection of active cells:\u003c\/strong\u003e enables identification of respiring bacteria via intracellular formazan formation\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFluorescent signal generation:\u003c\/strong\u003e reduced CTC forms a fluorescent formazan detectable by microscopy\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRapid assay performance:\u003c\/strong\u003e measurable activity can be observed within minutes under appropriate conditions\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSupports quantitative workflows:\u003c\/strong\u003e used for enumeration of viable, metabolically active cells\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplicable to diverse sample types:\u003c\/strong\u003e suitable for environmental, water, food, and microbiological samples\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch3\u003eTypical Applications\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eMicrobial viability and activity assessment\u003c\/strong\u003e\u003cbr\u003eCTC is widely used to identify metabolically active bacteria by detecting respiratory activity at the single-cell level.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnvironmental and water sample analysis\u003c\/strong\u003e\u003cbr\u003eUsed for epifluorescent microscopic enumeration of respiring bacteria in environmental and aquatic systems.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMarine microbiology studies\u003c\/strong\u003e\u003cbr\u003eApplied in quantitative methodologies for measuring metabolic activity in marine bacterial populations.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCellular redox activity assays\u003c\/strong\u003e\u003cbr\u003eUtilized in studies evaluating electron transport chain activity and cellular respiration in both prokaryotic and eukaryotic systems.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3\u003eFunctional Role\u003c\/h3\u003e\n\u003cp\u003eCTC functions as a tetrazolium-based redox probe that is reduced by components of the cellular electron transport chain. This reduction produces an insoluble formazan precipitate that accumulates within actively respiring cells. The resulting fluorescent signal allows for direct visualization and quantification of metabolically active cells using fluorescence microscopy techniques.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3\u003eFluorescence Properties\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eFormazan fluorescence (solid state)\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExcitation maximum:\u003c\/strong\u003e 450 nm\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEmission maximum:\u003c\/strong\u003e 630 nm\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch3\u003ePerformance Notes\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eCTC reduction is associated with active cellular respiration and electron transport activity\u003c\/li\u003e\n\u003cli\u003eSignal intensity may vary depending on organism type, metabolic state, and assay conditions\u003c\/li\u003e\n\u003cli\u003eTypical assays yield measurable activity within short incubation periods under appropriate conditions\u003c\/li\u003e\n\u003cli\u003eCTC-based methods are commonly used for quantitative and comparative microbial activity studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch3\u003eHandling \u0026amp; Storage\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eStore according to product specifications and label instructions.\u003c\/li\u003e\n\u003cli\u003eProtect from light to preserve dye integrity and fluorescence performance.\u003c\/li\u003e\n\u003cli\u003eHandle using appropriate laboratory safety practices.\u003c\/li\u003e\n\u003cli\u003eConsult the SDS for detailed handling, storage, and safety information.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cp\u003eClick a question to expand.\u003c\/p\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eWhat is Cyanoditolyl Tetrazolium Chloride (CTC) used for?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cp\u003eCTC is used to measure cellular respiration and metabolic activity by identifying actively respiring cells through formation of a fluorescent formazan product.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eHow does CTC detect active cells?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cp\u003eCTC is reduced by components of the electron transport chain in metabolically active cells, forming a fluorescent formazan that accumulates intracellularly and can be detected by microscopy.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eWhat types of samples can be analyzed with CTC?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cp\u003eCTC is commonly used with environmental, water, marine, and food samples, as well as cultured microbial populations.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eWhat microscopy methods are used with CTC?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cp\u003eCTC labeling is typically analyzed using epifluorescence microscopy and related fluorescence imaging techniques.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eWhat are the fluorescence properties of CTC?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cp\u003eThe reduced formazan product exhibits fluorescence with an excitation maximum near 450 nm and an emission maximum near 630 nm.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/div\u003e","brand":"Polysciences","offers":[{"title":"19292-1 (1 g)","offer_id":46425716687062,"sku":"19292-1","price":1539.0,"currency_code":"USD","in_stock":true},{"title":"19292-100 (100 mg)","offer_id":46425716719830,"sku":"19292-100","price":235.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0713\/5258\/2358\/files\/NEW_19292_ctc_ecoli2_1_1_d539d378-f213-4a3f-b309-7f49b7df2a67.png?v=1738253029"},{"product_id":"tb-fluorostain-kit","title":"TB Fluorostain Kit","description":"\u003cp\u003eFluorescent detection of M. tuberculosis and other acid-fast bacteria. Fluorochrome staining of mycobacteria in an acid-fast smear offers several advantages over traditional carbol-fuchsin methods. Lower magnification is required; Mycobacteria, including\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eM. tuberculosis, M. leprae, M. avium\u003c\/em\u003e-intracellular fluoresce orange-yellow on a dark black background.\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eM. tuberculosis\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eappears as slightly curved or straight rods with rounded ends. The rods will be 0.3 to 0.6μm in length.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e100 tests per kit\u003c\/li\u003e\n\u003cli\u003eLess than 12 minutes to perform staining\u003c\/li\u003e\n\u003cli\u003eMicrowave method reduces time and enhances results\u003c\/li\u003e\n\u003cli\u003eMuch less Auramine O and Rhodamine B used, reducing costs and hazards\u003c\/li\u003e\n\u003cli\u003eFaster, superior counterstain\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eFDA approved for\u003cspan\u003e \u003c\/span\u003e\u003cem\u003ein vitro\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003ediagnostic use in the United States\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit contains:\u003c\/strong\u003e\u003cbr\u003e500ml of Staining Solution A\u003cbr\u003e500ml of Acid Solution B\u003cbr\u003e500ml Counterstaining Solution C\u003c\/p\u003e","brand":"Polysciences","offers":[{"title":"22422-1 (1 kit)","offer_id":46425723404502,"sku":"22422-1","price":316.23,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0713\/5258\/2358\/files\/22422tb-fluorostain-kit_ed082c0b-8336-449e-924d-1a4a4de194c7-358919.jpg?v=1737984161"},{"product_id":"afb-ziehl-neelson-kit-hot-method","title":"AFB Ziehl-Neelsen Kit (Hot Method)","description":"\u003cp\u003eBacteriological stain kit used to identify acid-fast organisms, mainly Mycobacteria. Helpful in diagnosing Mycobacterium tuberculosis since its lipid rich cell wall makes it resistant to Gram stain.Can also be used to stain other bacteria like Nocardia. Kit contains a primary dye (carbol-fuchsin), a decolorizer (acid-alcohol) and a counter stain (methylene blue). Decolorizer is used to decolorize the bacteria that \"dislike\" the primary stain. Counter stain is used to stain those bacteria which were decolorized by the acid alcohol. Acid-fast bacilli will stain bright red, while non-acid fast organisms will stain blue or green.Kit includes: Carbol Fuchsin (Ziehl-Neelson), Acid Alcohol, and Methylene Blue (8 oz. bottle of each)\u003c\/p\u003e","brand":"Polysciences","offers":[{"title":"24669-1 (1 kit)","offer_id":46425738805462,"sku":"24669-1","price":255.68,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0713\/5258\/2358\/files\/24669_21c2331f-d76a-43c1-912a-ab8bddf12905-335970.jpg?v=1737982752"},{"product_id":"neat-stain-gram-stain-kit","title":"Gram Stain Set (Stabilized)","description":"\u003cp\u003eFor staining bacteria from cultures or specimens by the differential Gram stain method. Gram Stain sets have been specifically designed for stat procedures and\/or laboratories using manual staining methods. This kit is used for in vitro diagnostic use.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Contains:\u003c\/strong\u003e\u003cbr\u003e     A.) 2 oz. of Crystal Violet\u003cbr\u003e     B.) 2 oz. of Stabilized Iodine\u003cbr\u003e     C.) 2 oz. of Decolorizer\u003cbr\u003e     D.) 2 oz. of Safranin\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eNeat\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e- Self-contained foil sealed, pre-filled multi-well containers\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFast\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e- Immerse slides sequentially in each reagent for specified time, air dry and read\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConvenient\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e- Additional foils for re-sealing containers\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Polysciences","offers":[{"title":"25036-1 (1 kit)","offer_id":46425754763478,"sku":"25036-1","price":136.43,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0713\/5258\/2358\/files\/25036_3eab2ae8-7b99-4c5a-ad8c-31d2cf1880f7-283036.jpg?v=1737983249"},{"product_id":"psvue-794-reagent-kit","title":"PSVue® 794 Reagent Kit","description":"\u003cp\u003eA near-infrared fluorescent probe for detection of apoptotic cells, bacteria and other anionic membranes. The PSVue® 794 reagent kit contains components to provide a 1 mM solution of PSVue®794 in aqueous solution.\n\u003cbr\u003eKey Features of PSVue® Probes:\u003c\/p\u003e\n\n\u003cp\u003e  Bind to a variety of cell types which have negatively chargedphospholipids exposed on their membranes including apoptoticcells [1], necrotic cells [2], Gram+ and Gram- bacteria [3-5], activated cells, tumor vascular endothelial cells, viruses, etc.\n\u003cbr\u003e  Available in a range of detection wavelengths from long-UV tonear infrared.\n\u003cbr\u003e  Suitable for in vitro and in vivo use.\n\u003cbr\u003e  Suitable for high-throughput screening assays.\n\u003cbr\u003e  Bind to the same PS site as annexin-V.\u003c\/p\u003e\n\n\u003cp\u003eKit Contains: Vial containing pre-weighted amount of apo-PSS794 solid dye(at least 1 mg), Vial of diluent X (1 ml), Vial of 4.2mM zinc nitrate solution in diluents X (1 ml)\n\u003cbr\u003eNote: Absolute ethanol is needed to formulate the product but not provided.\u003c\/p\u003e","brand":"Polysciences","offers":[{"title":"25101-1 (1 kit)","offer_id":46425758990550,"sku":"25101-1","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0713\/5258\/2358\/files\/NEW_25101_b6d112b9-5872-4787-9b52-fb4a3105be78.png?v=1738252774"},{"product_id":"psvue-380-reagent-kit","title":"PSVue® 380 Reagent Kit","description":"\u003cp\u003eA long wavelength ultraviolet fluorescent probe for detection of apoptotic cells, bacteria, and other anionic membranes.\n\u003cbr\u003eKey Features of PSVue® Probes:\u003c\/p\u003e\n\n\u003cp\u003e  Bind to a variety of cell types which have negatively chargedphospholipids exposed on their membranes including apoptoticcells [1], necrotic cells [2], Gram+ and Gram- bacteria [3-5], activated cells, tumor vascular endothelial cells, viruses, etc.\n\u003cbr\u003e  Available in a range of detection wavelengths from long-UV tonear infrared.\n\u003cbr\u003e  Suitable for in vitro and in vivo use.\n\u003cbr\u003e  Suitable for high-throughput screening assays.\n\u003cbr\u003e  Bind to the same PS site as annexin-V.\u003c\/p\u003e\n\n\u003cp\u003eThe PSVue® 380 (formerly PSS-380) reagent kit contains components to provide a 1 mM solution of PSVue® 380 in aqueous solution.\n\u003cbr\u003eKit Contains: Vial containing pre-weighed amount of apo-PSS380 solid dye (at least 0.5 mg) and vial of 8.4 mM zinc nitrate solution in water (0.5 mL)\n\u003cbr\u003eNote: Absolute ethanol is required to formulate the product but is not provided.\u003c\/p\u003e","brand":"Polysciences","offers":[{"title":"25102-1 (1 kit)","offer_id":46425759252694,"sku":"25102-1","price":438.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0713\/5258\/2358\/files\/NEW_25102_5eb6298e-936b-426d-8679-69b01249f28f.png?v=1738252774"},{"product_id":"psvue-biotin","title":"PSVue® Biotin","description":"\u003cp\u003eVial contains 1mg of solid. PSVue® Biotin can be complexed with streptavidin-coated quantum dots (not provided) for in vivo and in vitro use. Procedures to formulate PSVue® biotin and prepare the PSVue® biotin-streptavidin-coated quantum dot complex are provided.\u003c\/p\u003e","brand":"Polysciences","offers":[{"title":"25683-1 (1 kit)","offer_id":46425774620886,"sku":"25683-1","price":340.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0713\/5258\/2358\/files\/logo_only_fbf4142e-aba7-4a9f-bc79-d3ac59d69498-281307.jpg?v=1737983982"},{"product_id":"psvue-550","title":"PSVue® 550","description":"\u003cp\u003eThe PSVue® 550 reagent kit contains components to provide ~0.5 mL of 1 mM solution of PSVue® 550. The compound has an absorbance max at 553nm and fluorescence emission max at 615nm.\u003c\/p\u003e","brand":"Polysciences","offers":[{"title":"25684-1 (1 kit)","offer_id":46425774686422,"sku":"25684-1","price":467.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0713\/5258\/2358\/files\/logo_only_f8daa946-80f3-4b3f-bf4b-ffbd6984f5db-227084.jpg?v=1737983982"},{"product_id":"psvue-643","title":"PSVue® 643","description":"\u003cp\u003eThe PSVue643® kit contains 0.25mL of a 1mM solution of PSVue643® in water. The compound has an absorbance max at 643nm and a fluorescence emission max at 658nm.\u003c\/p\u003e","brand":"Polysciences","offers":[{"title":"25685-1 (1 kit)","offer_id":46425774948566,"sku":"25685-1","price":420.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0713\/5258\/2358\/files\/logo_only_ad9d4f61-87e7-407e-8026-0fb7cf0145f9-881968.jpg?v=1737983982"},{"product_id":"afb-kinyoun-kit-cold-method","title":"AFB Kinyoun Kit (Cold Method)","description":"\u003cp\u003eMethod of staining acid-fast microorganisms, specifically mycobacterium. Procedure is similar to Ziehl-Neelsen stain, but does not involve heating the slides.\n\u003cbr\u003eKinyoun staining method uses carbol-fuchsin as a primary stain, followed by decolorization with an acid-alcohol solution and methylene blue as a counterstain. Kinyoun carbol-fuschsin has a greater concentration of phenol and basic fuchsin and does not require heating in order to stain properly.\n\u003cbr\u003eWhen viewed under a microscope, Kinyoun stained slides will show acid-fast organisms as red and non acid-fast organisms as blue.\n\u003cbr\u003eKit includes: Carbol Fuchsin (Kinyoun), Acid Alcohol, and Methylene Blue (8 oz. bottle of each)\u003c\/p\u003e","brand":"Polysciences","offers":[{"title":"25765-1 (1 kit)","offer_id":46425776095446,"sku":"25765-1","price":333.41,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0713\/5258\/2358\/files\/25765_e4e13e1f-8135-439d-b7c4-63b891bedc5c-786157.jpg?v=1737982752"}],"url":"https:\/\/polysciences.com\/collections\/microbiology-stain-kits.oembed","provider":"Polysciences","version":"1.0","type":"link"}