⟡ EM Buffers / Osmium Tetroxide Fixatives / Electron Microscopy
2,4,6-sym-Collidine Buffer Kit, EM Grade is a biologically stable buffering system designed for osmium tetroxide and other electron microscopy fixatives. The buffer system provides improved buffering capacity and stability compared to traditional veronal acetate buffer systems used in EM specimen preparation workflows.
This kit is commonly used in electron microscopy laboratories for preparation of buffered fixative solutions during biological specimen processing and fixation procedures.
Key Features & Benefits
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Biologically stable buffer system: suitable for EM fixation workflows
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Designed for osmium tetroxide: compatible with osmium-based fixatives
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Improved buffering performance: provides greater stability than traditional veronal acetate buffers
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EM grade formulation: intended for electron microscopy specimen preparation
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Convenient kit format: includes components required for buffer preparation
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Large preparation volume: one kit prepares approximately 1000 mL of buffer
Product Details
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Product name: 2,4,6-sym-Collidine Buffer Kit
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Grade: EM Grade
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Buffer type: Biologically stable collidine buffer system
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Applications: Osmium tetroxide fixation, EM fixatives, electron microscopy specimen preparation
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Final preparation volume: Approximately 1000 mL buffer per kit
Kit Contents
- 5 × 5.34 mL Collidine solution
- 5 × 18 mL 1.0 N HCl
Typical Applications
Electron microscopy fixation
Used in EM specimen preparation workflows requiring stable buffering systems for biological fixation procedures.
Osmium tetroxide preparation
Commonly used with osmium tetroxide fixative formulations for electron microscopy applications.
Biological specimen processing
Suitable for tissue preparation and fixation workflows in microscopy laboratories.
Research laboratory workflows
Used in EM laboratories requiring reliable buffering systems with improved stability characteristics.
Functional Properties
2,4,6-sym-Collidine buffer systems are designed to provide stable buffering performance during electron microscopy fixation procedures. Compared to traditional veronal acetate buffers, collidine systems may offer improved buffering capacity and biological stability in EM workflows.
The kit format simplifies preparation of larger buffer volumes for routine specimen processing and fixation applications.
Why Use 2,4,6-sym-Collidine Buffer Kit?
This EM grade buffer kit is widely used for electron microscopy fixation workflows requiring stable buffer systems compatible with osmium tetroxide and related fixatives.
Its improved buffering performance and convenient preparation format make it useful for biological EM specimen preparation and laboratory processing applications.
Handling & Storage
- Store kit components according to product labeling and SDS instructions.
- Handle acids and fixative-related materials using appropriate laboratory protective equipment.
- Use in properly ventilated laboratory environments.
- Prepare buffers according to established EM laboratory procedures.
- Consult the SDS for complete handling, storage, and safety information.
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FAQ
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What is 2,4,6-sym-Collidine Buffer Kit used for?
It is used as a biologically stable buffering system for osmium tetroxide and other EM fixatives during electron microscopy specimen preparation.
Why is collidine buffer used in EM workflows?
Collidine buffers provide improved stability and buffering capacity compared to traditional veronal acetate buffer systems.
What does the kit contain?
The kit contains 5 × 5.34 mL collidine solution and 5 × 18 mL 1.0 N HCl.
How much buffer does one kit prepare?
One kit prepares approximately 1000 mL of buffer solution.
Is this kit intended for electron microscopy applications?
Yes. This EM Grade kit is designed for electron microscopy fixation and specimen preparation workflows.
