⟡ Electrophoresis Reagents / Polyacrylamide Gels / IEF Applications
Acrylamide, Ultra Pure Grade is a high-purity electrophoresis reagent used to prepare polyacrylamide gels for separation of nucleic acid fragments and proteins. It is suitable for precision gels used in electrophoresis and isoelectric focusing.
Because of its low acidity, this acrylamide helps minimize ampholyte binding, supporting fine pH gradient formation in IEF applications. Fluorescence-free gels are readily prepared, and this Ultra Pure grade may be used for gel scanning in the UV region where very low baseline noise is required.
Key Features & Benefits
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Ultra Pure acrylamide: suitable for precision electrophoresis gel preparation
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PAGE applications: used for separation of nucleic acid fragments and proteins
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IEF compatibility: supports fine pH gradient formation in isoelectric focusing
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Low acidity: helps reduce ampholyte binding during gel preparation
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Fluorescence-free gels: suitable for applications requiring low background signal
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UV scanning: helps produce gels with very low baseline noise in the UV region
Product Details
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Product name: Acrylamide, Ultra Pure Grade
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Purity: >99.9%
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Specific conductance: 2 µmho/cm for 35% (w/v) solution
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Applications: Electrophoresis, PAGE, isoelectric focusing, fluorescence-free gel preparation, and UV gel scanning
Typical Applications
Polyacrylamide gel electrophoresis
Used to prepare gels for electrophoretic separation of nucleic acid fragments and proteins.
Isoelectric focusing
Suitable for precision gels where fine pH gradients are required.
Fluorescence-free gel preparation
Used to prepare gels for applications where low fluorescence background is important.
UV gel scanning
Supports preparation of gels with very low baseline noise for scanning in the UV region.
Functional Properties
Acrylamide is polymerized to form polyacrylamide gel matrices used in electrophoretic separation workflows. The Ultra Pure grade is designed for applications where low acidity and low background signal are important to gel performance.
In isoelectric focusing, reduced ampholyte binding helps support stable pH gradient formation. For UV scanning applications, this grade helps produce gels with very low baseline noise.
Why Use Acrylamide, Ultra Pure Grade?
Acrylamide, Ultra Pure Grade is selected for precision electrophoresis and isoelectric focusing workflows that require high-purity gel reagents and consistent gel performance.
Its suitability for fluorescence-free gel preparation and UV gel scanning makes it useful for researchers working with nucleic acid, protein, and pH-gradient separation applications.
Handling & Storage
- Handle using appropriate laboratory safety procedures.
- Avoid inhalation, ingestion, and skin contact.
- Use appropriate personal protective equipment during handling.
- Store according to product label and SDS guidance.
- Consult the SDS for complete safety, storage, and disposal information.
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FAQ
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What is Acrylamide, Ultra Pure Grade used for?
It is used to prepare polyacrylamide gels for electrophoretic separation of nucleic acid fragments and proteins.
Is this acrylamide suitable for isoelectric focusing?
Yes. It is suitable for precision gels used in isoelectric focusing and fine pH gradient formation.
What is the listed purity?
The listed purity is >99.9%.
What is the specific conductance?
The specific conductance of a 35% (w/v) solution is listed as 2 µmho/cm.
Where can I buy Acrylamide, Ultra Pure Grade?
Polysciences supplies Acrylamide, Ultra Pure Grade for electrophoresis, isoelectric focusing, and gel preparation workflows.
