Anti-Human IgG (H&L) in sheep

(Secondary antibodies and conjugates, affinity purified) We recommend that each investigator determine the actual optimum dilution for a given antibody or antibody conjugate for each specific assay used. Enzyme conjugates of affinity purified antibodies are supplied with lot-specific recommended dilutions. Products without specifically stated dilutions should be used according to the information below.

Conjugate
ELISA
Blotting
Flow
Tissue






Flourescein
nd
nd
1:50-1:200
1:200-1:1000


Rhodamine, Texas Red
nd
nd
1:50-1:200
1:200-1:1000


Biotin
1:5k-1:20k
1:3k-1:10k
nd
1:100-1:1000


Peroxidase
1:40k-1:10k
1:2k-1:5k
nd
1:200-1:2000


Alkaline phosphatase
1:1k-1:2k
1:500-1:1k
nd
1:100-1:1000

liquid