⟡ Histology Stains / Plasma Stains / Microscopy Dyes
Biebrich Scarlet, C.I. 26905 is a laboratory staining dye widely used as a counterstain and plasma stain in histology, pathology, and microscopy workflows. Also known as Acid Red 66 or Ponceau B, this red dye supports tissue differentiation and contrast enhancement in multi-step staining procedures.
With a reported λ max of 505 nm, Biebrich Scarlet is commonly selected for histological staining applications requiring strong red coloration, reliable tissue visualization, and compatibility with connective tissue and plasma staining protocols.
Key Features & Benefits
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Histology stain: widely used in tissue staining and microscopy applications
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Counterstaining dye: improves contrast in multi-step staining workflows
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Plasma stain: useful for selective red staining of plasma and tissue components
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Strong red coloration: supports tissue differentiation and visualization
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Research laboratory use: suitable for pathology, histology, and microscopy protocols
Product Details
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Product name: Biebrich Scarlet
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Color index: C.I. 26905
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Alternative names: Acid Red 66; Ponceau B
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Application: Histology stain and counterstain
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Typical use: Plasma staining and tissue visualization
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λ max: 505 nm
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Research areas: Histology, pathology, microscopy, and laboratory staining protocols
Typical Applications
Histology staining
Used in tissue staining protocols requiring strong red coloration and contrast enhancement for microscopic evaluation.
Counterstaining workflows
Frequently incorporated into multi-step staining methods to improve differentiation of tissue structures and cellular components.
Plasma staining
Useful as a plasma stain in laboratory protocols involving connective tissue and cytoplasmic visualization.
Microscopy & pathology research
Supports staining applications across pathology, life science research, and microscopy laboratories.
Functional Properties
Biebrich Scarlet provides strong red staining characteristics that support tissue contrast and microscopic visualization in histological workflows.
The dye is commonly used in research and diagnostic staining applications where reproducible coloration and compatibility with established staining protocols are required.
Why Use Biebrich Scarlet?
Biebrich Scarlet remains a widely used laboratory staining dye because of its effectiveness as a counterstain and plasma stain across histology and microscopy applications.
Researchers and laboratory professionals frequently select Biebrich Scarlet for tissue staining workflows requiring vivid coloration, reliable staining performance, and compatibility with established histological methods.
Handling & Storage
- Store in a cool, dry environment away from direct light and moisture.
- Keep container tightly sealed when not in use.
- Avoid contamination during laboratory handling procedures.
- Use appropriate laboratory protective equipment during handling.
- Consult the SDS for complete safety and handling information.
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FAQ
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What is Biebrich Scarlet used for?
Biebrich Scarlet is commonly used as a histology stain, counterstain, and plasma stain in microscopy and pathology workflows.
What are other names for Biebrich Scarlet?
Biebrich Scarlet is also known as Acid Red 66 and Ponceau B.
What is the λ max of Biebrich Scarlet?
Biebrich Scarlet has a reported absorption maximum (λ max) of 505 nm.
Is Biebrich Scarlet used in histology?
Yes. Biebrich Scarlet is widely used in histology and tissue staining protocols requiring strong red coloration and tissue contrast.
Where can I buy Biebrich Scarlet stain?
Polysciences supplies Biebrich Scarlet, C.I. 26905 for laboratory staining, histology, microscopy, and research applications.
