Cedarwood oil

Cedarwood oil is used as an immersion medium for light microscopy and as a clearing agent for microscope sections. In oil-immersion microscopy, an appropriate immersion medium provides the optical connection between the coverslip and an objective designed for oil immersion, supporting efficient light collection at high numerical aperture.

Polysciences Cedarwood Oil, Cat. 04851, is supplied as a pale yellow liquid for laboratory microscopy and histology workflows.

Key Properties

• Product: Cedarwood Oil
• Catalog number: 04851
• CAS Number: 68990-83-0
• Appearance: Pale yellow liquid
• Specific gravity at 25°C: 0.94700–0.95900
• Storage: Room temperature

Applications

Light Microscopy

Used as an immersion oil with microscope objectives designed for oil immersion. Immersion media reduce the refractive-index discontinuity between the coverslip and objective, allowing high-numerical-aperture objectives to collect light efficiently.

Microscope Section Clearing

May be used as a clearing medium for microscope sections during histological specimen preparation. Cedarwood oil has a long history of use in workflows where optical clearing of prepared sections is required before microscopic examination.

Microscopy Use Considerations

• Use only with microscope objectives intended for oil immersion
• Follow the microscope or objective manufacturer's recommendations for immersion-medium compatibility
• Avoid mixing different immersion oils unless compatibility has been established
• Clean the objective according to the microscope manufacturer's recommended procedure after use

Handling & Storage

• Store at room temperature in a cool, well-ventilated location
• Protect from light
• Keep away from heat, hot surfaces, sparks, open flames, and other ignition sources
• Avoid contact with skin and eyes
• Wear appropriate protective gloves and eye protection during handling

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Safety & Documentation

Cedarwood Oil is classified in the current Polysciences SDS as a combustible liquid that causes skin and eye irritation. Use appropriate laboratory ventilation and personal protective equipment, and consult the current Safety Data Sheet (SDS) before handling.