CellVue® Red Mini Kit is a fluorescent cell membrane labeling kit designed to incorporate a lipophilic red dye into lipid regions of cell membranes. The dye contains long aliphatic tails that support stable membrane labeling for cell-tracking and membrane-labeling research.
The kit provides CellVue® Red as a 1 mM dye stock solution in ethanol together with a dedicated cell-labeling diluent. CellVue® Red has an excitation maximum of 567 nm and an emission maximum of 588 nm.
Labeling patterns may vary with cell type, membrane composition, dye concentration, and staining conditions. Researchers should optimize and validate the protocol for the cells, detection platform, and experimental workflow being used.
Key Features
- Lipophilic fluorescent dye for cell membrane labeling
- Supports stable labeling of membrane lipid regions
- Excitation maximum: 567 nm
- Emission maximum: 588 nm
- Supplied as a convenient mini kit
- Product number: 25567
Kit Contents
- CellVue® Red dye stock: 1 vial containing 0.1 mL of a 1 × 10-3 M solution in ethanol
- Cell-labeling diluent: 1 vial containing 10 mL
Applications
Cell Membrane Labeling
CellVue® Red is used to fluorescently label lipid regions of cell membranes for detection by compatible fluorescence instruments.
Cell-Tracking Research
The stable membrane-associated signal may be used in research workflows that monitor labeled cells over the duration of a validated experiment.
Multiparameter Fluorescence Studies
The red fluorescence profile may support combination with appropriately selected probes when instrument configuration, compensation, and spectral overlap are evaluated before use.
Isolated Cell & Organ Research
CellVue® Red has been used in cell-tracking research involving isolated organ preparations. Researchers should establish application-specific labeling and detection conditions.
Labeling & Detection Considerations
- Labeling intensity and distribution may vary by cell type and membrane composition
- Optimize dye concentration, cell concentration, incubation time, and wash conditions for each application
- Confirm compatibility with the available excitation source and emission filters
- Include appropriate unstained and single-color controls in multiparameter experiments
Instrument settings and fluorescence controls should be established using the specific cell type, labeling protocol, and detection platform planned for the study.
Application Tips
- Prepare labeling solutions according to the validated laboratory protocol
- Mix cells gently and thoroughly to promote uniform exposure to the dye
- Protect the dye and labeled samples from unnecessary light exposure
- Evaluate cell condition and labeling performance after staining
- Use suitable controls when combining CellVue® Red with other fluorescent probes
Handling & Storage
- Keep kit components securely closed when not in use
- Protect fluorescent reagents from unnecessary light exposure
- Use clean laboratory technique to prevent contamination
- Follow the product Safety Data Sheet and laboratory-specific handling procedures
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FAQ
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1. What is the CellVue® Red Mini Kit used for?
The kit is used to fluorescently label lipid regions of cell membranes for cell-tracking, membrane-labeling, and compatible fluorescence-detection research.
2. What are the excitation and emission wavelengths of CellVue® Red?
CellVue® Red has an excitation maximum of 567 nm and an emission maximum of 588 nm.
3. What does the CellVue® Red Mini Kit contain?
The kit contains one vial with 0.1 mL of 1 mM CellVue® Red dye stock in ethanol and one vial containing 10 mL of cell-labeling diluent.
4. How does CellVue® Red label cell membranes?
The dye contains long aliphatic tails that associate with lipid regions of the cell membrane, producing a membrane-associated fluorescent signal.
5. Can CellVue® Red be used with other fluorescent probes?
It may be used in multiparameter fluorescence studies when the selected probes, filters, instrument settings, compensation, and spectral overlap are evaluated before use.
6. Will every cell type stain the same way?
No. Labeling intensity and distribution may vary with cell type, membrane composition, cell concentration, dye concentration, and staining conditions. The protocol should be optimized for each application.
Safety & Documentation
Use standard laboratory safety practices and review the product Safety Data Sheet before handling the kit. Wear appropriate personal protective equipment and follow institutional procedures for handling fluorescent dyes and ethanol-containing solutions.
