CHAPS, 98%
CHAPS is a specialized zwitterionic detergent commonly used in laboratory workflows for the preparation of biological
samples. It is frequently selected to solubilize membrane proteins and lipids while helping preserve protein structure,
making it useful for protein extraction, membrane protein studies, and biochemical research applications.
CHAPS is widely used for extracting membrane-associated proteins because it can disrupt lipid bilayers while generally
minimizing protein denaturation under appropriate conditions. It is often described as a non-denaturing detergent and is
used in workflows where maintaining native-like protein behavior is important, including certain functional studies and
enzymatic assays. In biochemistry and biology applications, CHAPS is also used as an alternative to Triton X-100 in some
protocols.
Key Properties
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Detergent type: Zwitterionic detergent
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Typical use: Solubilization of membrane proteins and lipids in biological sample preparation
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Behavior: Commonly used as a “non-denaturing” detergent in compatible protocols
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Grade: 98% (as labeled)
Applications
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Protein extraction: Solubilization of membrane-associated proteins from biological samples
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Membrane protein research: Studies requiring detergent-based disruption of lipid bilayers
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Biochemical and molecular biology workflows: Sample preparation where protein structure retention is desirable
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Method substitution studies: Used in some protocols as an alternative to Triton X-100 (method-dependent)
Synonyms / Acronyms
- CHAPS
- 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate
FAQs
Common questions about CHAPS, 98%.
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What is CHAPS used for in the lab?
CHAPS is commonly used for biological sample preparation, especially for solubilizing membrane proteins and
lipids during extraction and analysis workflows.
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What does “zwitterionic detergent” mean?
Zwitterionic detergents contain both positive and negative charges in the same molecule. This can support
effective solubilization while helping minimize unwanted protein unfolding in some systems.
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Is CHAPS considered non-denaturing?
CHAPS is often described as non-denaturing because it is used in protocols intended to preserve protein
structure and activity. Actual outcomes depend on concentration, buffer composition, temperature, and the
specific protein.
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Can CHAPS be used instead of Triton X-100?
In some biochemistry and biology workflows, CHAPS is used as an alternative to Triton X-100. Whether it is a
suitable substitute depends on the assay, target, and required solubilization strength.
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What does “98%” indicate for this product?
“98%” refers to the labeled grade/purity specification for the detergent material. Refer to your internal
specifications and documentation for the applicable test method and acceptance criteria.
