⟡ Protein Stains / SDS-PAGE Dyes / Electrophoresis Reagents
Coomassie® Blue R250, C.I. 42660 is a rapid-acting protein stain commonly used for visualization of proteins in SDS-PAGE and polyacrylamide gel electrophoresis applications. The dye provides sensitive staining performance for protein detection in electrophoresis workflows.
Also known as Brilliant Blue R250 and Acid Blue 83, this dye is widely used in molecular biology, biochemistry, and protein analysis laboratories for gel staining and protein visualization procedures.
Key Features & Benefits
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Rapid protein staining: suitable for SDS-PAGE gel visualization workflows
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Sensitive dye formulation: supports protein detection in electrophoresis gels
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Widely used laboratory stain: common in molecular biology and biochemistry applications
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Compatible with polyacrylamide gels: suitable for PAGE and SDS-PAGE procedures
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Multiple recognized names: also known as Brilliant Blue R250 and Acid Blue 83
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Strong visible absorbance: λmax approximately 588 nm
Product Details
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Product name: Coomassie® Blue R250
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C.I. number: 42660
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CAS number: 6104-59-2
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Alternative names: Brilliant Blue R250; Acid Blue 83
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λmax: 588 nm
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Applications: SDS-PAGE staining, protein visualization, electrophoresis, polyacrylamide gel staining
Typical Applications
SDS-PAGE protein staining
Commonly used for visualization of protein bands in SDS-polyacrylamide gel electrophoresis workflows.
Polyacrylamide gel electrophoresis
Suitable for staining PAGE gels used in protein separation and analysis procedures.
Protein analysis workflows
Used in molecular biology and biochemistry laboratories for protein detection and gel documentation.
Research laboratory applications
Frequently utilized in protein purification, analytical electrophoresis, and teaching laboratory workflows.
Functional Properties
Coomassie® Blue R250 binds to proteins within polyacrylamide gels to produce visible blue-stained protein bands following electrophoretic separation.
The dye is widely used because it provides relatively rapid staining and sensitive visualization for routine protein electrophoresis applications.
Why Use Coomassie® Blue R250?
Coomassie® Blue R250 is commonly selected for protein gel staining because it provides reliable visualization of proteins separated by SDS-PAGE and related electrophoresis techniques.
Its widespread use in molecular biology and biochemical laboratories makes it a standard staining reagent for routine protein analysis workflows.
Handling & Storage
- Store in a cool, dry laboratory environment.
- Keep containers tightly sealed when not in use.
- Handle using appropriate laboratory protective equipment.
- Avoid unnecessary exposure to dust or staining solutions.
- Consult the SDS for complete safety, storage, and handling information.
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FAQ
Click a question to expand.
What is Coomassie® Blue R250 used for?
It is used for staining proteins in SDS-PAGE and polyacrylamide gel electrophoresis applications.
Is Coomassie® Blue R250 suitable for SDS gels?
Yes. It is commonly used as a rapid and sensitive stain for SDS-PAGE protein gels.
What are alternative names for this dye?
Coomassie® Blue R250 is also known as Brilliant Blue R250 and Acid Blue 83.
What is the λmax of Coomassie® Blue R250?
The listed absorbance maximum is approximately 588 nm.
What is the CAS number for Coomassie® Blue R250?
The CAS number is 6104-59-2.
