Crystal Violet C.I. 42555, certified, is a biological stain used in Gram staining, histological staining, cytology, and cell-based laboratory staining applications.
Synonyms: Crystal Violet, Gentian Violet, Basic Violet 3, C.I. 42555.
Crystal Violet is commonly used where strong visual contrast is required for microscopy and laboratory staining workflows. It is widely recognized as the primary stain in Gram staining procedures and may also be used in selected cell and tissue staining methods.
Researchers and laboratory professionals use Crystal Violet in microbiology, histology, cytology, and cell biology workflows where a dependable certified stain is required.
Key Properties
- Certified biological stain
- C.I. 42555 dye
- Commonly used as the primary stain in Gram staining
- Used in microbiology, histology, and cytology workflows
- Produces strong violet coloration for microscopy applications
- Suitable for laboratory staining and research use
Applications
Gram Staining
Crystal Violet is widely used as the primary stain in Gram staining procedures for bacterial differentiation.
Histology & Cytology
The dye may be used in laboratory staining workflows involving tissue sections, cells, and microscopic evaluation.
Cell-Based Assays
Crystal Violet is commonly used in selected cell staining and cell viability-related assay workflows where stained cell material is measured or visualized.
General Laboratory Staining
Certified Crystal Violet is useful for research and quality-controlled staining applications requiring a recognized biological dye.
Compatibility & Bonding Considerations
- Commonly used in aqueous or alcohol-based staining preparations
- Compatible use depends on the staining protocol and formulation
- Performance may vary with concentration, fixation method, and sample type
- Follow validated laboratory protocols for intended use
Final staining intensity and contrast depend on formulation, sample preparation, staining time, differentiation steps, and microscopy method.
Application Tips
- Prepare staining solutions according to the applicable protocol
- Use clean glassware and appropriate laboratory-grade solvents
- Filter solutions if particulate clarity is required
- Validate concentration and staining time for each sample type
Handling & Storage
- Store tightly sealed in a cool, dry environment
- Protect from unnecessary light and moisture exposure
- Avoid inhalation of dust and contact with skin or eyes
- Follow standard chemical handling procedures
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FAQ
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1. What is Crystal Violet C.I. 42555 used for?
Crystal Violet C.I. 42555 is used as a biological stain in Gram staining, histology, cytology, and selected cell staining workflows.
2. Is Crystal Violet used in Gram staining?
Yes. Crystal Violet is commonly used as the primary stain in Gram staining procedures.
3. What are common synonyms for Crystal Violet?
Common names include Crystal Violet, Gentian Violet, Basic Violet 3, and C.I. 42555.
4. Can Crystal Violet be used for cell staining?
Yes. Crystal Violet is commonly used in selected cell staining and cell-based assay workflows, depending on the validated protocol.
5. How should Crystal Violet be handled?
Handle Crystal Violet using standard laboratory precautions, avoid dust exposure, and refer to the Safety Data Sheet for complete guidance.
Safety & Documentation
Use Crystal Violet with appropriate laboratory safety precautions. Refer to the product Safety Data Sheet (SDS) for complete handling, storage, and safety guidance.
