Estradiol Sepharose® 4FF

Sepharose® 6B affinity chromatography beads covalently modified with steroids or other ligands such that ligand binding to receptors is not compromised resulting in high receptor-binding specificity.

Efficient isolation and purification of receptor proteins (nuclear and others)
Ligand binding affinity studies
Preparation of receptor-ligand complexes for crystallography studies.
Assisting in the structure-based design of receptor selective ligands (e.g. see Manas et al.)

Typical ligand loading: 10-14 μmoles/mL bead.
Sold under license from PTI Research, Inc. to MTTI and distributed by Polysciences, Inc. Sepharose is a trademark of GE Healthcare.