⟡ Histology & Microscopy / Biological Fixatives
Glutaraldehyde Solution, 50% (Biological Grade)
Glutaraldehyde Solution, 50% is a high-concentration, biological grade glutaraldehyde fixative used in microscopy, histology, and general laboratory fixation workflows. This solution is purified to help minimize by-product formation and support long-term stability, making it suitable for demanding applications where consistency and reproducibility are important.
Biological grade glutaraldehyde is commonly selected for morphology studies and routine fixation procedures. For applications requiring higher purity and extended stability, EM grade glutaraldehyde may be preferred. Polysciences glutaraldehyde solutions are manufactured and quality-controlled to reduce undesirable polymeric impurities that can affect fixation performance.
Key Features & Benefits
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50% glutaraldehyde solution: high-concentration stock solution for dilution and custom fixation protocols
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Biological grade: purified to minimize by-product formation and support reliable laboratory use
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Designed for morphology studies: suitable for routine fixation and structural preservation workflows
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Reduced polymeric impurities: supports consistent fixation performance across experiments
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Long-term stability: formulated to maintain quality during refrigerated storage
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Flexible use: can be diluted to working concentrations depending on application requirements
Product Details
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Product name: Glutaraldehyde Solution, 50%
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Grade: Biological grade
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CAS number: 111-30-8
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Product number: 00377
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Form: Aqueous solution
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Concentration: 50% (w/v)
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Storage: Store refrigerated at 2–8°C
Typical Applications
Histology and tissue fixation
Glutaraldehyde is widely used to fix biological tissues by crosslinking proteins, helping preserve cellular and structural detail for microscopy-based analysis.
Microscopy sample preparation
Biological grade glutaraldehyde is used in preparation workflows for light microscopy and related imaging techniques where consistent fixation is required.
Morphological studies
Commonly used in studies focused on maintaining structural integrity of cells and tissues during processing and analysis.
General laboratory fixation workflows
This 50% stock solution can be diluted to working concentrations for a wide range of biological and materials research applications.
Functional Role
Glutaraldehyde is a dialdehyde fixative that reacts with primary amine groups in proteins and other biomolecules, forming covalent crosslinks. This crosslinking stabilizes cellular structures and reduces degradation during sample processing. The result is improved preservation of morphology for downstream imaging and analytical techniques.
Why Use a 50% Glutaraldehyde Stock Solution
A 50% glutaraldehyde solution provides flexibility for researchers who require precise control over fixation conditions. By diluting to the desired working concentration, users can tailor fixation strength, penetration, and reaction time to match specific sample types and experimental goals.
Handling & Storage
- Store refrigerated at 2–8°C.
- Keep container tightly closed when not in use.
- Handle using appropriate personal protective equipment, including gloves and eye protection.
- Use in a well-ventilated area or chemical fume hood.
- Consult the SDS for detailed safety, handling, and disposal information.
FAQ
Click a question to expand.
What is Glutaraldehyde Solution, 50% used for?
This product is used as a high-concentration fixative stock solution for histology, microscopy, and general laboratory fixation workflows.
What does biological grade glutaraldehyde mean?
Biological grade glutaraldehyde is purified to reduce by-products and impurities, making it suitable for routine laboratory fixation and morphology studies.
Can 50% glutaraldehyde be diluted for use?
Yes. This solution is typically diluted to lower working concentrations depending on the application and sample requirements.
What is the CAS number for glutaraldehyde?
The CAS number for glutaraldehyde is 111-30-8.
How should Glutaraldehyde Solution, 50% be stored?
This product should be stored at 2–8°C in a tightly sealed container. Refer to the SDS for complete storage guidance.
When should EM grade glutaraldehyde be used instead?
EM grade glutaraldehyde is typically selected for applications requiring higher purity and more stringent control of impurities, such as electron microscopy and certain immunochemical techniques.
