⟡ Electron Microscopy / Histology & Immunochemical Fixatives
Glutaraldehyde, EM Grade, 25% is a high-purity aqueous glutaraldehyde solution for electron microscopy, histochemical, and immunological techniques. This concentrated EM grade fixative is designed for laboratories that need reliable fixation performance, consistent purity, and flexibility when preparing working-strength glutaraldehyde solutions.
Polysciences was the first to develop a superior grade of glutaraldehyde suitable for immunochemical techniques. Each batch of vacuum-distilled glutaraldehyde manufactured by Polysciences is analyzed to maintain consistent quality and help minimize contaminants that may affect antigenicity or sample integrity.
Key Features & Benefits
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25% EM grade glutaraldehyde: concentrated aqueous solution for preparation of working fixative concentrations
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Recommended for histochemical and immunological techniques: suitable for demanding microscopy and sample preparation workflows
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Vacuum-distilled quality: manufactured and analyzed for consistent batch-to-batch performance
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Supports antigen preservation: designed to help reduce contaminants that can interfere with antigenicity
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Low polymeric impurity profile: Polysciences EM grade glutaraldehyde has been reported to show practically no contamination by undesirable polymeric products
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Flexible format options: available in ampoules and bottle formats to support different laboratory workflows
Product Details
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Product name: Glutaraldehyde, EM Grade, 25%
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Product number: 01909
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CAS number: 111-30-8
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Concentration: 25% aqueous solution
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Available sizes: 01909-10 (10 x 10 mL ampoules), 01909-100 (100 mL bottle), 01909-5 (5 x 100 mL bottles)
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Storage: Store at 4°C
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Handling: Gloves, chemical goggles, and fume hood recommended
Typical Applications
Electron microscopy fixation
Glutaraldehyde is widely used as a primary fixative in electron microscopy sample preparation because it rapidly crosslinks proteins and helps preserve ultrastructural detail.
Histochemical techniques
EM grade glutaraldehyde is used in histochemical workflows where consistent fixation and preservation of cellular or tissue architecture are required.
Immunological and immunochemical techniques
Polysciences EM grade glutaraldehyde is designed for applications where contaminant control and preservation of antigenicity are important considerations.
Preparation of working fixative solutions
The 25% concentration provides a convenient stock solution for laboratories preparing lower working concentrations for microscopy and biological sample preparation.
Functional Role
Glutaraldehyde is a dialdehyde fixative that reacts primarily with amine-containing biomolecules, including proteins. Through covalent crosslinking reactions, it stabilizes biological structures during fixation and processing. In electron microscopy and histology workflows, this crosslinking helps preserve fine structural detail for downstream imaging and analysis.
Why Choose 25% EM Grade Glutaraldehyde?
A 25% EM grade glutaraldehyde solution offers a useful balance of concentration, purity, and handling flexibility. Researchers can dilute the stock solution to the desired working concentration while relying on EM grade purity for applications where fixation quality, reproducibility, and reduced impurity levels matter.
In an independent quality control study, unopened refrigerated Polysciences glutaraldehyde ampoules showed no polymers and no impurities at 235 nm even after four years of storage.
Handling & Storage
- Store at 4°C.
- Keep container tightly closed until use.
- Use appropriate personal protective equipment, including gloves and chemical goggles.
- Handle in a chemical fume hood.
- Consult the SDS and product specification sheet before handling, preparing, or using this product.
FAQ
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What is Glutaraldehyde, EM Grade, 25% used for?
Glutaraldehyde, EM Grade, 25% is used as a high-purity fixative stock solution for electron microscopy, histochemical, immunological, and immunochemical techniques.
Why use 25% glutaraldehyde instead of a lower concentration?
The 25% concentration provides a convenient stock solution that can be diluted to working concentrations required by specific microscopy or fixation protocols.
What does EM grade glutaraldehyde mean?
EM grade glutaraldehyde refers to a high-purity glutaraldehyde solution suitable for electron microscopy and related sample preparation workflows where low impurity levels and consistent fixation performance are important.
What is the CAS number for glutaraldehyde?
The CAS number for glutaraldehyde is 111-30-8.
How should Glutaraldehyde, EM Grade, 25% be stored?
This product should be stored at 4°C. Refer to the SDS and product specification sheet for complete storage and handling guidance.
What precautions should be used when handling glutaraldehyde?
Glutaraldehyde should be handled with appropriate personal protective equipment, including gloves and chemical goggles, and used in a chemical fume hood. Always review the SDS before use.
