Granular Blue (GB) Dihydrochloride

Granular Blue (GB) is a fluorescent retrograde tracer used in experimental studies of the peripheral nervous system to determine the number and origin of neurons projecting to a specific area. The retrograde tracing techniques allow for detailed assessment of neuronal connections from a single population of neurons to their various targets throughout the nervous system. These techniques allow the "mapping" of connections between neurons in a particular structure (e.g. the eye) and the target neurons in the brain.

In rats, GB is effectively transported over long distances to produce blue fluorescent labeling of neuronal cytoplasm in cell bodies and proximal dendrites. It also produces an accumulation of silver-blue granules in cytoplasm.

Excitation: 365 nm
Emission: 410 nm