Indocyanine green

Indocyanine Green (ICG) is a tricarbocyanine dye with strong absorption in the near-infrared spectral region. Cat. 08263 is used as a laboratory reagent and in research involving hemodynamic measurements, optical characterization, and near-infrared fluorescence.

Synonyms: ICG; Cardiogreen; Cardio-Green; Fox Green.

Key Properties

• Catalog number: 08263
• CAS Number: 3599-32-4
• Chemical class: Tricarbocyanine dye
• Molecular formula: C43H48N2O6S2·Na
• Molecular weight: 774.99 g/mol
• Physical form: Dark green crystalline solid
• λmax: 775 nm
• UV extinction coefficient: >23.0 × 10⁴ L/mol·cm at 784 ± 1 nm in methanol
• Hygroscopic: Yes
• Package size: 50 mg
• Storage: Room temperature

Applications

Hemodynamic Research

Indocyanine Green is used as an optical dye in hemodynamic research. Its strong near-infrared absorption enables optical detection in experimental workflows involving changes in dye concentration over time.

Near-Infrared Fluorescence Research

ICG is widely studied as a near-infrared fluorescent dye. Its absorption and fluorescence behavior makes it useful in experimental optical-imaging and fluorescence-detection research.

Spectroscopic Studies

The strong near-infrared absorbance of ICG supports spectroscopic characterization and optical-method development. Cat. 08263 has an extinction-coefficient requirement of greater than 23.0 × 10⁴ L/mol·cm at 784 ± 1 nm in methanol.

Optical & Experimental Considerations

• Spectral behavior depends on solvent, concentration, and aggregation state
• The 775 nm λmax and the extinction-coefficient assay at 784 ± 1 nm represent different measurement conditions and should not be treated as interchangeable values
• Maintain consistent solvent composition and dye concentration when comparing absorbance or fluorescence measurements
• Account for concentration-dependent aggregation when interpreting optical spectra
• Use experimentally measured spectra under the intended assay conditions when selecting excitation, detection, or filtering parameters

Application Tips

• Prepare concentrations consistently when comparing optical or fluorescence measurements
• Record solvent composition, dye concentration, optical path length, and measurement wavelength for quantitative spectroscopy
• Minimize unnecessary exposure to ambient humidity during weighing and preparation because the material is hygroscopic
• Validate absorbance and fluorescence behavior under the specific solvent and concentration conditions used in the experimental method

Handling & Storage

• Store at room temperature in a cool, well-ventilated location
• Minimize exposure to ambient moisture during handling
• Use adequate laboratory ventilation
• Avoid contact with skin and eyes
• Wear protective gloves, safety glasses, and suitable protective clothing

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Safety & Documentation

Indocyanine Green can cause skin irritation. Use appropriate laboratory ventilation and wear protective gloves, safety glasses, and suitable protective clothing during handling. Review the current product documentation before use.

• Product Specification – Indocyanine Green, Cat. 08263
• Safety Data Sheet (SDS) – Indocyanine Green, Cat. 08263