Nile Blue Methacrylamide, ≥ 80%

Nile Blue dye itself is a fluorescent dye that is used routinely in histology to impart a blue color to cell nuclei, where it highlights the distinction between neutral lipids (triglycerides, etc.) which are stained pink and fatty acids, which are stained blue.Nile Blue Methacrylamide has been used to covalently link Nile Blue dye into the backbone of polymers for use in various sensor applications. Chandrasekharan, et al have developed a plasticizer-free autoclavable ratiometric alcohol sensor based on Nile Blue Methacrylamide incorporated into a hydrogel polymer backbone.1 The film formed from the Nile Blue hydrogel exhibits both absorption and fluorescence changes upon contact with ethanol, and the fluorescence λ max decreases with the increase of ethanol concentration. The sensor can therefore be used to accurately determine ethanol concentration in a system.In addition, Pham, et al have developed a polymeric nanostructured material incorporating Nile Blue methacrylamide into the polymer, and this material is being developed for the recording of biometric features for anti-counterfeiting applications.2
Purity: >85% (HPLC relative absorption from λ=254 nm); ca. 90% based on 1H NMRUV Absorption and fluorescence emission maxima of Nile Blue in different solvents3

Solvent
Absorption λmax(nm)
Emission λmax(nm)


Toluene
493
574


Acetone
499
596


DMF
504
598


Ethanol
628
667


Water
635
674


1.0 N HCI (pH=1.0)
457
556


0.1 N NaOH (pH=11.0)
522
668

* Excitation and emission data is for Nile Blue dye itself.
Store: in dark at 0° C