PEG di(NHS), Mp ~3000 (26114)
PEG di(NHS) (NHS-PEG-NHS) is a bifunctional polyethylene glycol derivative
bearing N-hydroxysuccinimide (NHS) ester groups on both termini.
These activated esters are designed for efficient amide bond formation
with primary amines under mild reaction conditions.
With an average molecular weight of approximately 3,000 Da,
this homobifunctional PEG linker provides a relatively short, flexible spacer
that enables controlled crosslinking and conjugation of amine-containing
biomolecules. PEG di(NHS), Mp ~3000 is commonly used in bioconjugation,
hydrogel synthesis, and surface functionalization where closer spacing between
linked components is desired.
Key Characteristics
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PEG backbone: Hydrophilic spacer that improves solubility and compatibility in aqueous systems
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Reactive groups: NHS esters for selective reaction with primary amines
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Functionality: Homobifunctional (NHS groups on both ends)
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Average molecular weight: Mp ~3,000
Typical Applications
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Protein crosslinking & PEGylation:
Forms stable amide bonds with lysine residues to modify proteins and peptides,
enhancing solubility and stability.
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Hydrogel & polymer synthesis:
Crosslinks amine-functional polymers to generate PEG-based networks with
tunable mechanical properties.
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Surface coating & biocompatibility:
Enables PEGylation of surfaces, nanoparticles, and biosensors to reduce
nonspecific adsorption.
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Drug delivery research:
Functionalizes carriers and scaffolds where shorter PEG spacing is beneficial
for controlled interactions.
FAQs
Common questions about PEG di(NHS), Mp ~3000.
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What is the advantage of a PEG linker with Mp ~3000?
A PEG of approximately 3,000 Da provides a shorter spacer length,
allowing closer proximity between conjugated molecules while still
maintaining PEG-associated hydrophilicity.
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Which functional groups react with NHS esters?
NHS esters react primarily with primary amines, such as those found on
lysine residues in proteins or amine-terminated polymers.
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Is this material intended for clinical or GMP use?
PEG di(NHS) is typically supplied for research and development use.
Suitability for regulated or clinical applications should be evaluated
by the end user.
