PEG di(NHS), Mp ~6000 (26115)
PEG di(NHS) (NHS-PEG-NHS) is a bifunctional polyethylene glycol derivative functionalized with N-hydroxysuccinimide (NHS) ester groups on both termini. These activated esters are designed to react efficiently with primary amines, forming stable amide bonds under mild conditions.
With an average molecular weight of approximately 6,000 Da, this homobifunctional PEG linker enables controlled crosslinking and spacing between amine-containing molecules. PEG di(NHS) is widely used in bioconjugation, hydrogel formation, and surface modification where predictable reactivity and PEG-mediated hydrophilicity are desired.
Key Properties
-
PEG backbone: Hydrophilic, flexible spacer that improves solubility and biocompatibility
-
Reactive groups: NHS esters for efficient amide bond formation with primary amines
-
Functionality: Homobifunctional (NHS on both termini)
-
Average molecular weight: Mp ~6,000
Applications
-
Protein crosslinking & PEGylation: Forms amide bonds with lysine residues to enhance solubility, stability, and circulation behavior of biomolecules.
-
Hydrogel & polymer synthesis: Crosslinks amine-functional polymers and biomacromolecules to form well-defined PEG-based networks.
-
Surface modification & antifouling coatings: Introduces PEG layers on surfaces, nanoparticles, and biosensors to reduce nonspecific adsorption.
-
Drug delivery research: Functionalizes carriers and scaffolds for improved dispersion and performance in experimental delivery systems.
FAQs
Common questions about PEG di(NHS), Mp ~6000.
-
What does “di(NHS)” mean?
“di(NHS)” indicates that the PEG molecule is functionalized with NHS ester groups on both ends, making it homobifunctional and suitable for crosslinking two amine-containing species.
-
What types of groups react with NHS esters?
NHS esters primarily react with primary amines, such as those found on lysine residues of proteins or amine-functional polymers, to form stable amide bonds.
-
Why use a PEG linker with Mp ~6000?
A PEG of approximately 6,000 Da provides a balance between flexibility, spacing, and solubility, allowing controlled separation between conjugated components without excessive steric bulk.
-
Is PEG di(NHS) suitable for regulated or clinical use?
PEG di(NHS) is typically supplied for research and development use. Users should assess suitability for their specific application and regulatory requirements.
