Polybead® Carboxylate Microspheres, 6.00 µm (17141)
Polybead® Carboxylate Microspheres are monodisperse polystyrene microspheres
functionalized with surface carboxyl groups. These reactive carboxyl groups
enable covalent coupling of proteins, peptides, and other biomolecules to the
bead surface using established conjugation chemistries.
This product is supplied as a 2.5% solids (w/v) aqueous suspension
and features a nominal mean diameter of 6.00 µm. Larger-diameter
microspheres are commonly selected for applications requiring ease of handling,
optical visibility, and robust surface functionalization.
Nominal Reference Values
-
Mean particle diameter: 6.00 µm
-
Solids content: 2.5% (w/v) aqueous suspension
-
Coefficient of Variance (CV): ~10%
-
Particle concentration: ~2.10 × 108 particles/mL
Typical Applications
-
Protein and biomolecule coupling:
Carboxyl-functional surfaces support covalent attachment for assay development,
binding studies, and method optimization.
-
Immunoassays and diagnostics research:
Used as solid supports in particle-based assay formats where controlled size and
surface chemistry are important.
-
Cell interaction and binding studies:
Larger microspheres are often selected for visibility and manipulation in
microscopy-based workflows.
-
Calibration and method development:
Applied in particle counting, sizing, and flow-based techniques during
instrument or protocol evaluation.
FAQs
Common questions about Polybead® Carboxylate Microspheres, 6.00 µm.
-
What does “6.00 µm” particle size indicate?
A 6.00 µm mean diameter places these microspheres in the larger
microparticle range, which can simplify handling, visualization, and
separation compared with submicron particles.
-
What are carboxylate microspheres used for?
Carboxylate-functional beads are commonly used for covalent coupling of
biomolecules in research applications such as immunoassays, binding
studies, and surface chemistry development.
-
What does CV mean for particle size?
CV (coefficient of variance) describes the width of the particle size
distribution around the mean. A CV of ~10% indicates a relatively narrow,
monodisperse population. Refer to the COA for lot-specific values.
-
How are these microspheres supplied?
This product is supplied as a 2.5% (w/v) aqueous suspension. Gentle mixing
is typically recommended prior to use to ensure uniform dispersion.
-
Are these beads suitable for clinical or GMP use?
Polybead® Carboxylate Microspheres are generally intended for research and
development use. Users should evaluate suitability for their specific
application and regulatory requirements.
