Poly(L-lysine) hydrobromide is a cationic polypeptide derived from the amino acid L-lysine. Supplied as a 0.1% aqueous solution, this polymer provides positively charged amine functionality that supports electrostatic interaction with negatively charged biomolecules, cells, and surfaces.
Related names: Poly-L-lysine hydrobromide, poly(L-lysine) HBr, PLL HBr, polylysine hydrobromide, and L-lysine homopolymer hydrobromide.
This material has a molecular weight range of approximately 60,000–100,000 and is commonly used in research involving cell culture surface coating, gene delivery and bioconjugation workflows, tissue engineering studies, and drug-delivery formulation development.
Key Properties
- Cationic polypeptide derived from L-lysine
- Product Number: 09730
- CAS Number: 25988-63-0
- Synonym: PLL HBr
- Molecular weight: approximately 60,000–100,000
- Supplied as a 0.1% solution in water
- Contains positively charged amine functionality
- Supports electrostatic interaction with negatively charged biomolecules and surfaces
Applications
Gene Delivery & Bioconjugation
Poly(L-lysine) hydrobromide is used in genetic research and bioconjugation workflows where cationic polymer interactions with negatively charged DNA, RNA, proteins, or surfaces are relevant.
Cell Culture & Tissue Engineering
Poly-L-lysine coatings are widely used to promote cell attachment to cultureware, glass, and biomaterial surfaces. The cationic polymer surface can support electrostatic interaction with cells and extracellular matrix components.
Drug Delivery Systems
This cationic polypeptide is used in research involving biodegradable carrier formulations and drug-delivery system development where polymer charge, biomolecule interaction, and aqueous compatibility are important.
Solubility & Compatibility Considerations
- Supplied as a 0.1% aqueous solution
- Compatible use depends on substrate, coating method, contact time, and downstream workflow
- Electrostatic interactions may vary with pH, ionic strength, buffer composition, and biomolecule charge
- Users should confirm compatibility with cells, biomolecules, surfaces, media, buffers, and formulation components before use
Final performance depends on coating concentration, surface chemistry, incubation conditions, rinsing procedure, drying conditions, and the intended cell culture or formulation workflow.
Application Tips
- Use clean cultureware, glass, or biomaterial surfaces when preparing coatings
- Evaluate coating concentration, contact time, and rinsing conditions for the intended cell type or surface
- Control pH and ionic strength when studying electrostatic interactions with biomolecules
- Confirm suitability in small-scale trials before routine or scaled use
Handling & Storage
- Store at 4°C
- Use gloves and a fume hood when handling
- Keep container tightly closed when not in use
- Use standard laboratory handling practices and appropriate personal protective equipment
- Follow the product SDS for complete handling, storage, cleanup, and disposal guidance
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FAQ
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1. What is Poly(L-lysine) hydrobromide?
Poly(L-lysine) hydrobromide is a cationic polypeptide derived from L-lysine. Its positively charged amine groups support electrostatic interaction with negatively charged biomolecules, cells, and surfaces.
2. What is PLL HBr used for?
PLL HBr is used in research involving cell culture surface coating, gene delivery and bioconjugation workflows, tissue engineering studies, and drug-delivery formulation development.
3. How is this product supplied?
This product is supplied as a 0.1% solution in water. It has a molecular weight range of approximately 60,000–100,000.
4. Why is poly-L-lysine used for cell culture coating?
Poly-L-lysine provides a positively charged coating that can improve interaction between negatively charged cell surfaces and culture substrates such as glass or plastic.
5. How should this product be stored and handled?
Store at 4°C. Use gloves and a fume hood when handling, and review the Safety Data Sheet before use for complete handling, storage, cleanup, and disposal guidance.
Safety & Documentation
Review the product specification and Safety Data Sheet before use. Handle with gloves, a fume hood, and appropriate laboratory precautions. Follow SDS guidance for storage, cleanup, and disposal.
