Safranin O, certified by the Biological Stain Commission

Safranin O is commonly used in routine histology, cytology, and specialized staining techniques. It produces a bright red to orange coloration, providing excellent contrast and differentiation of various cellular and tissue elements. It is particularly effective in staining plant tissues, cartilage, and certain cellular structures, enhancing the visibility of microscopic specimens.

Safranin-O and Fast Green staining are commonly used for distinguishing between different tissue types and in Gram staining to identify bacterial cells.