Silica Microspheres, 0.15 µm (24320)
Inorganic supports such as silica microspheres are used across a range of research workflows,
including nucleic acid isolation, cell separation, and immuno- and DNA-based assays. Silica
substrates are valued for their flexible silanization chemistries and typically low nonspecific
binding of proteins compared with many polymeric supports.
This item is a uniform, non-porous silica (SiO2) microsphere product with a
nominal mean diameter of 0.15 µm (150 nm). Product lines in this family are commonly offered
across nominal diameters from ~150 nm to 5 µm, with typical size CVs in the ~10–15% range
(confirm exact lot values on the COA for your shipment).
Key Properties
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Material: Silica (SiO2)
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Particle type: Non-porous microspheres
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Nominal mean diameter: 0.15 µm (150 nm)
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Surface chemistry: Compatible with silanization/functionalization workflows
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Typical size uniformity (family guidance): CV often ~10–15% (verify lot-specific COA)
Typical Applications
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Nucleic acid isolation workflows:
Silica supports are commonly used in research protocols where surface chemistry and handling of small particles are important.
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Immuno- and DNA-based assay development:
Used as an inorganic particle platform that can be functionalized via established silane chemistries for assay prototyping.
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Cell separation and sample preparation research:
Applied in method development where inorganic microspheres are preferred for stability and surface modification options.
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Low-background imaging/labeling method development:
Often selected when low autofluorescence and controlled surface functionalization are desired (validate for your instrument and labels).
FAQs
Common questions about Silica Microspheres, 0.15 µm (150 nm).
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What does “0.15 µm” mean in practical terms?
0.15 µm is 150 nm. This product’s nominal mean diameter is in the sub-micron range,
which can be helpful for applications that benefit from high surface area and fine particle size.
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Why use silica microspheres instead of polymer microspheres?
Silica is an inorganic substrate that is commonly chosen for its surface chemistry options
(silanization) and for use cases where an inorganic particle platform is preferred. Selection
depends on your assay chemistry, buffers, and instrumentation.
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What is “silanization” and why is it mentioned?
Silanization is a common way to modify silica surfaces using silane reagents to introduce
functional groups (for example, amine, epoxy, or other reactive handles). The best choice of
reagent and protocol depends on your target molecule and downstream workflow.
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What does CV mean for particle size?
CV (coefficient of variance) is a measure of distribution width (uniformity) around the mean size.
Lower CV generally indicates a tighter size distribution. For lot-specific size data, rely on the COA.
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How should I handle and store silica microspheres?
Handling depends on how the product is supplied (e.g., dry powder vs. suspension) and your protocol.
In general, keep containers tightly closed, avoid contamination, and follow the product documentation
(SDS/COA) for storage conditions and any recommended dispersion steps.
