Super Decalcifier I: Delicate is recommended for delicate specimens that require fast diagnostic work, including IHC, cytochemistry, and special stain procedures.
Recommended use: Mildly calcified specimens such as bone marrow biopsies, core biopsies, and other delicate tissue specimens.
This ready-to-use decalcifier is designed for human and animal tissue workflows where preservation of detail is important. It may be used for IHC, special stains, and routine staining procedures.
Decalcification time depends on specimen size, density, and endpoint requirements. Specimens should be checked regularly during processing.
Key Properties
- Ready-to-use decalcifying solution
- Recommended for delicate specimens
- May decalcify in as little as 3 hours, depending on specimen type
- Recommended for IHC, special stains, and routine stains
- Supports enhanced nuclear detail
- Environmentally safe and biodegradable
Applications
Bone Marrow Biopsies
Recommended for mildly calcified bone marrow biopsy specimens, including core biopsies.
IHC & Special Stains
Super Decalcifier I: Delicate is recommended for immunohistochemistry, cytochemistry, special stain, and routine stain procedures.
Human & Animal Tissue
The ready-to-use formulation may be used with human and animal tissue specimens requiring gentle decalcification.
Suggested Decalcification Times
- Bone marrow biopsies: 15–30 minutes
- Small cancellous bone: 2–4 hours
- Femur wedge: 3–5 hours
- Mature bone, 1 cm: 4–6 hours
- Whole femur or teeth: overnight
The standard procedure is to check specimens every ½–1 hour for mildly calcified specimens and every 1–2 hours for compact bone to determine the endpoint of decalcification.
Preparation & Handling Notes
- Rinse specimens thoroughly in running tap water after decalcification
- Do not mix with formalin
- Avoid contact with metal forceps and metal surfaces
- Wear gloves and protective clothing during decalcification procedures
- Perform procedures under a fume hood
- Store specimen in 70% ethyl alcohol until ready to process
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FAQ
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1. What is Super Decalcifier I: Delicate used for?
It is used for decalcifying delicate specimens, including mildly calcified bone marrow biopsies and specimens intended for IHC, cytochemistry, special stains, or routine stains.
2. Is this product ready to use?
Yes. Super Decalcifier I: Delicate is supplied as a ready-to-use decalcifying solution.
3. How quickly does it decalcify specimens?
Suggested times vary by specimen type. Bone marrow biopsies may require 15–30 minutes, while larger or denser specimens may require several hours or overnight processing.
4. Can it be used before IHC staining?
Yes. Super Decalcifier I: Delicate is recommended for immunohistochemistry techniques.
5. Should specimens be rinsed after decalcification?
Yes. Specimens should be rinsed thoroughly in running tap water after decalcification.
Safety & Documentation
Use appropriate laboratory safety precautions. Wear gloves and protective clothing, avoid contact with metal forceps and metal surfaces, and perform procedures under a fume hood. Refer to the product Safety Data Sheet (SDS) for complete handling, storage, and safety guidance.
