ViaCheck™ Viability Control 0% is a suspension of microspheres that simulate dead or dying cells stained with Trypan Blue. This pre-stained, non-biological control is designed for image-based cell viability analyzers that rely on the Trypan Blue dye exclusion method.
Supplied as a 20 mL suspension, ViaCheck™ Viability Control 0% provides a nominal 0% viable control with an approximate bead concentration of ~1 x 106 beads/mL. Lot-specific concentration and viability values are reported on the Certificate of Analysis and may be used to support routine instrument QC and performance monitoring.
Key Properties
- 0% viability control for image-based cell viability analyzer QC
- Simulates dead or dying cells stained with Trypan Blue
- Pre-stained microsphere reference material
- Non-biological surrogate for live/dead cell viability workflows
- Product Number: 24622
- Size: 20 mL
- Nominal viability: 0%
- Approximate bead concentration: ~1 x 106 beads/mL
- Supplied in buffered salts and surfactant containing 0.08% sodium azide
- Lot-specific values reported on the Certificate of Analysis
Applications
Cell Viability Analyzer QC
Used to support routine maintenance and QC programs for image-based cell viability analyzers, helping confirm consistent instrument performance over time.
Trypan Blue Dye Exclusion Workflows
Designed for instruments that rely on the Trypan Blue dye exclusion method to confirm live/dead ratios and counts.
Instrument Validation & Performance Monitoring
May be used to validate image-based viability instruments before commissioning and to monitor ongoing analyzer performance using lot-specific COA values.
Inter-Instrument Comparability
Useful for facilities comparing performance across multiple viability analyzers or establishing QC criteria based on historical instrument performance.
Compatibility & Instrument Considerations
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Instrument type: Image-based cell viability analyzers
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Detection method: Trypan Blue dye exclusion
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Example platforms: Vi-CELL™, CEDEX, HiRes, Countess™, and related image-based viability instruments
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Control type: Pre-stained microsphere suspension
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Performance factors: Results may depend on instrument settings, sample handling, mixing, pipetting, and user-defined control limits
Use the lot-specific Certificate of Analysis to establish expected values and determine appropriate QC pass/fail criteria for the instrument and workflow.
Preparation Tips
- Mix the vial thoroughly before sampling to ensure a well-dispersed suspension.
- Work carefully and promptly when sampling and pipetting to minimize settling effects.
- Pipet the required sample volume according to the analyzer method or site-specific procedure.
- Use the analyzer settings recommended in the technical data sheet as a starting point, then optimize as needed for the specific instrument.
- Refer to the lot-specific Certificate of Analysis when setting expected values and QC acceptance criteria.
Handling & Storage
- Store refrigerated at 2-8°C.
- Do not permit to freeze.
- Freezing may result in irreversible particle aggregation.
- Keep container tightly closed when not in use.
- Mix well before use and avoid prolonged standing after mixing.
- Use standard laboratory handling practices.
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Frequently Asked Questions
1. What is ViaCheck™ Viability Control 0% used for?
ViaCheck™ Viability Control 0% is used as a pre-stained microsphere reference material for image-based cell viability analyzer QC, validation, and performance monitoring.
2. What does 0% viability mean?
The 0% viability control simulates a sample of dead or dying cells stained with Trypan Blue and is used to confirm analyzer response for non-viable particles.
3. What instruments can ViaCheck™ controls be used with?
ViaCheck™ controls are intended for image-based viability instruments that rely on the Trypan Blue dye exclusion method, including platforms such as Vi-CELL™, CEDEX, HiRes, Countess™, and related analyzers.
4. What is the approximate bead concentration?
The nominal bead concentration is approximately ~1 x 106 beads/mL. Lot-specific concentration and viability values are provided on the Certificate of Analysis.
5. How should ViaCheck™ Viability Control 0% be stored?
Store refrigerated at 2-8°C and do not permit to freeze. Freezing may result in irreversible particle aggregation.
6. Is this product for clinical or diagnostic use?
No. This product is for research use only and is not intended for use in humans or for in vitro diagnostic use.
Safety & Documentation
This suspension contains sodium azide. Sodium azide may react with lead and copper plumbing to form explosive metal azides; flush disposal lines with a large volume of water when disposing of material. This product is for research use only and is not intended for use in humans or for in vitro diagnostic use. Refer to the Safety Data Sheet (SDS), Technical Data Sheet, and Certificate of Analysis for complete handling, storage, safety, and lot-specific guidance.
