Boc-amine PEG thiol, Mp 3000 (Boc-Amine-PEG-SH) is a heterobifunctional polyethylene glycol derivative with a thiol (-SH) group at one end and a Boc-protected amine at the other. These distinct end groups support sequential conjugation and surface-functionalization workflows in which the thiol can be reacted first while the amine remains protected for a later step.
Synonym: Boc-Amine-PEG-SH.
The terminal thiol can participate in thiol-maleimide conjugation and can also be used for attachment to compatible metal surfaces such as gold. After the thiol reaction is complete, the Boc protecting group can be removed under suitable acidic conditions to expose a primary amine for additional functionalization.
This Mp 3000 grade has a molecular-weight range of 2650–3350 Da, purity of ≥90%, and polydispersity of ≤1.1. Researchers requiring a longer PEG spacer can also consider Boc-amine PEG thiol, Mp 5000 or Boc-amine PEG thiol, Mp 10000. Additional end-group combinations are available in the Heterobifunctional PEGs collection.
Key Properties
- Heterobifunctional polyethylene glycol derivative
- Thiol (-SH) functionality at one terminus
- Boc-protected amine at the opposite terminus
- Molecular weight (Mp): 2650–3350 Da
- Purity: ≥90%
- Polydispersity: ≤1.1
- Appearance: white to yellowish powder
- Soluble in water, chloroform, DCM, DMF, and DMSO
Applications
Thiol-Maleimide Conjugation
Boc-Amine-PEG-SH can be used in conjugation workflows where the terminal thiol reacts with a maleimide-functionalized molecule. The Boc-protected amine remains masked during this step and can be exposed later for a second functionalization reaction.
Sequential Functionalization
The different terminal groups allow each end of the PEG chain to be addressed at a separate stage of a synthesis or conjugation workflow. This is useful when the thiol needs to react first while the amine remains protected until a later step.
Metal Surface Functionalization
The thiol terminus can be used in surface-modification workflows involving compatible metal substrates such as gold. The PEG chain provides spacing between the surface and the Boc-protected amine, which can later be deprotected for additional modification.
PEG Spacer Introduction
The approximately 3,000 molecular-weight PEG segment can be used as a hydrophilic spacer between conjugated components or between a surface and a terminal functional group. For applications requiring a longer PEG chain, compare the Mp 5000 and Mp 10000 grades.
Compatibility & Bonding Considerations
- Terminal thiol functionality can react with suitable maleimide-functionalized molecules
- Thiol functionality can be used for attachment to compatible metal surfaces such as gold
- The Boc group keeps the amine terminus protected during reaction steps where a free amine is not desired
- After Boc deprotection, the exposed primary amine can be used in a subsequent coupling step
- Soluble in water, chloroform, dichloromethane (DCM), dimethylformamide (DMF), and dimethyl sulfoxide (DMSO)
Conjugation efficiency depends on reagent concentration, solvent system, pH, accessibility of the thiol and reaction partner, and reaction time. Thiol-coupling and Boc-deprotection conditions should be selected for compatibility with the other molecules, surfaces, and functional groups in the system.
Application Tips
- Select a solvent system that keeps both the PEG derivative and the intended reaction partners in solution
- Limit unnecessary exposure of the thiol group to conditions that may promote oxidation before the intended coupling reaction
- Keep the Boc-protected amine intact until primary amine functionality is needed for the next reaction step
- Confirm that the selected Boc-deprotection conditions are compatible with the previously formed conjugate or modified surface
- Use analytical methods appropriate for the system to confirm coupling, deprotection, and subsequent functionalization
Handling & Storage
- Store at -20°C
- Keep the container tightly closed when not in use
- Store in a cool, well-ventilated location
- Use appropriate laboratory ventilation during handling
- Wear protective gloves, safety glasses, and suitable protective clothing
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FAQ
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1. What is Boc-Amine-PEG-SH used for?
Boc-Amine-PEG-SH is used in sequential conjugation and surface-functionalization workflows. The thiol terminus can participate in thiol-maleimide coupling or attachment to compatible metal surfaces, while the opposite amine remains Boc-protected for a later reaction step.
2. What functional groups are present on Boc-amine PEG thiol?
The PEG chain has a thiol (-SH) group at one end and a Boc-protected amine at the other. These different end groups allow each terminus to be used at a separate stage of a multistep conjugation or surface-modification workflow.
3. What are the molecular weight, purity, and polydispersity of the Mp 3000 grade?
Boc-amine PEG thiol, Mp 3000 has a molecular-weight range of 2650–3350 Da, purity of ≥90%, and polydispersity of ≤1.1. See the product specification for current quality requirements.
4. What solvents dissolve Boc-amine PEG thiol, Mp 3000?
Boc-amine PEG thiol, Mp 3000 is soluble in water, chloroform, dichloromethane (DCM), dimethylformamide (DMF), and dimethyl sulfoxide (DMSO).
5. What other molecular weights of Boc-Amine-PEG-SH are available?
Polysciences also offers Boc-amine PEG thiol, Mp 5000 and Boc-amine PEG thiol, Mp 10000. Choose the molecular weight based on the PEG spacer length and molecular architecture required for the application. For larger quantities, request a bulk quote.
Safety & Documentation
Store Boc-amine PEG thiol, Mp 3000 at -20°C and use appropriate laboratory ventilation, protective gloves, safety glasses, and suitable protective clothing during handling. See the Safety Data Sheet (SDS) for complete safety and handling information and the product specification for molecular weight, purity, appearance, and polydispersity. Additional end-group combinations are available in the Heterobifunctional PEGs collection.
